Expression of S100A14 is associated with cancer growth, invasion, and migration is well known. However, it is not known that subcellular location of S100A14 is correlated with cervical cancer, yet. This study investigated the role of S100A14 along with subcellular location in cervical cancer.
To begin with, the subcellular location of S100A14 was confirmed by immunohistochemistry in cervical cancer tissue microarray. The mRNA and protein expression of S100A14 were measured via qPCR and western blotting, and subcellular location of S100A14 was confirmed via immunocytochemistry in cervical cancer cell lines. Then the growth of a cervical cancer based on the expression of S100A14 was measured through stably expressed cervical cancer cells including over-expression and sh-RNA knockdown of S100A14.
The location of S100A14 according to progressing cancer was changed from membrane into cytosol in S100A14 stained cervical cancer tissue microarray. The subcellular location of S100A14 is into cytosol in cervical cancer HeLa and Caski cells, but is into membrane in immortal keratinocyte HaCaT cells. In the case of over-expressing S100A14, the cell growth is increased in HeLa and Caski cells. On the other hand, the cell growth is decreased in ME-180 cells while S100A14 expression was knock-down via over-expression of sh-S100A14.
The location of S100A14 expression was changed into cytosol from membrane while normal tissue in cervix progress to malignant cervical cancer. And, we verified that the control of S100A14 expression is associated with cancer growth in cervical cancer. These results suggest that S100A14 could be a feasible diagnostic marker via detecting location of expression and an effectual therapeutic target via controlled expression.