P17-12FOCALPOINT COMPUTER-ASSISTED PAP TEST SCREENING: VALIDATION TECHNOLOGY STUDY FOR IMPLEMENTATION IN A PUBLIC HEALTH SERVICE

08. Screening methods
A. Longatto-Filho 1, J.E. Levi 2, T.R. Martins 3, D. Dede Cohen 4, L.M. Fuza 4, L. Cury 4, L. Lina-Villa 3, J. Eluf-Neto 3.
1Laboratory of Medical Investigation (LIM14), Department of Pathology of Faculdade de Medicina da Universidade de São Paulo (USP's College of Medicine), São Paulo, Brazil (Brazil), 2Instituto de Medicina Tropical da Universidade de São Paulo (USP’s Institute of Tropical Medicine), Virology Laboratory; São Paulo, Brazil (Brazil), 3Faculdade de Medicina da Universidade de São Paulo (USP's College of Medicine), São Paulo, Brazil (Brazil), 4Fundação Oncocentro de São Paulo, São Paulo, Brazil (Brazil)

Background / Objectives

Pap test slides evaluated by computer-assisted methodology is believed to be more precise than manual reading. We validate the use of computer-assisted technology for Pap test reading for implementation in a public health service. 


Methods

We evaluated prospectively 12,084 PapTest from women examined at Fundação Oncocentro de São Paulo, with median age of 42 years old. The cervical samples were preserved in BD-SurePath liquid medium and prepared in BD Totallys equipment. The slides were primarily read by Focalpoint system (BD, Burlignton, USA) and afterwards 10 selected fields were revised by well-trained cytotechnologists and cytopathologists. 


Results

Manual slide reading identified 112 (0.9%) ASC-H, 329 (2.7%) LSIL, 70 (0.6%) HSIL and 2 (0.00%) invasive squamous cells carcinoma. Focalpoint quintiles classified the two invasive cancer in Q1 and almost 90% of HSIL and almost 85% of ASC-H in in Q1 and Q2, respectively. One HISL was classified as Q5 and detected in manual reading. Negative Pap test results were similarly distributed among all quintiles. Miscellaneous accounted 890 (7.4%). 


Conclusion

Computer-assisted screening can drastically reduce the need for manual Pap test reading, which may safely increase productivity.


References

1. Rebolj M, Rask J, van Ballegooijen M, Kirschner B, Rozemeijer K, Bonde J, Rygaard C, Lynge E. Cervical histology after routine ThinPrep or SurePath liquid-based cytology and computer-assisted reading in Denmark. Br J Cancer. 2015 Nov 3;113(9):1259-74.

2. Bowditch RC, Clarke JM, Baird PJ, Greenberg ML. Morphologic analysis of false negative SurePath® slides using Focalpoint™ GS computer-assisted cervical screening technology: An Australian experience. Diagn Cytopathol. 2015 Nov;43(11):870-8.


3. Stein MD, Fregnani JH, Scapulatempo-Neto C, Longatto-Filho A. Cervicovaginal cytology in patients undergoing pelvic radiotherapy using the Focalpoint system: results from the RODEO study. Diagn Pathol. 2015 Jan 16;10:1.

4. Stein MD, Fregnani JH, Scapulatempo C, Mafra A, Campacci N, Longatto-Filho A; RODEO Study Team From Barretos Cancer Hospital. Performance and reproducibility of gynecologic cytology interpretation using the FocalPoint system: results of the RODEO Study Team. Am J Clin Pathol. 2013 Oct;140(4):567-71.