SSim 01-06HLA CLASS II ANTIGEN EXPRESSION IN CERVICAL INTRAEPITHELIAL NEOPLASIA AND INVASIVE CANCER

04. Immunology
M. Sauer 1, M. Reuschenbach 1, N. Wentzensen 2, F. Soldano 3, B. Lahrmann 4, N. Grabe 5, D. Schmidt 6, M. Von Knebel Doeberitz 1, M. Kloor 1.
1Department of Applied Tumor Biology, Institute of Pathology, University of Heidelberg, and Clinical Coperation Unit, German Cancer Research Center (DKFZ), Heidelberg (Germany), 2Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Rockville, Maryland (United States), 3Massachusetts General Hospital, Harvard Medical School, Department of Surgery, Boston (United States), 4Hamamatsu Tissue Imaging and Analysis Center (TIGA), BIOQUANT, University of Heidelberg, Heidelberg (Germany), 5Hamamatsu Tissue Imaging and Analysis Center (TIGA), BIOQUANT, University of Heidelberg, and National Center of Tumor Diseases, Medical Oncology, University Hospital Heidelberg, University of Heidelberg, Heidelberg (Germany), 6Institute of Pathology, Viersen (Germany)

Background / Objectives

HLA class I antigen expression on tumor cells is essential for the recognition of tumor antigens by the immune system. HLA class II antigens normally are expressed by professional antigen-presenting cells, but are also reported to be expressed by several tumors of non-lymphoid origin. Strong HLA class II antigen expression has been described for a subset of HPV-associated cervical cancers. To characterize HLA class II antigen expression during HPV-induced cervical tumor development, we examined HLA class II antigen expression in CIN lesions and cervical cancers and correlated HLA class II expression with immune cell infiltration in the lesions and the adjacent stroma.


Methods

FFPE tissue sections of CIN1, CIN2, CIN3 and invasive SCC patients (n=103 in total) were analyzed by immunohistochemical staining with monoclonal antibodies specific for HLA class II antigens (LGII-612.14) and for different T cell markers (CD3, CD8, Foxp3, Granzyme B, CD3 zeta-chain).


Results

HLA class II antigen expression was absent in all samples of normal, non-neoplastic squamous cervical epithelium adjacent to lesions (n=29). However, a strong and uniform staining pattern was found in the columnar epithelium and cells of the squamocolumnar junction zone. HLA class II antigen expression was low in CIN1 (40.9%) and peaked in CIN2 (90.0%), then decreasing again towards CIN3 lesions (71.4%) and cancer (63.6%). In CIN3 and cancers high CD3+ and CD8+ lymphocyte infiltration correlated with lack of or heterogeneous HLA class II antigen expression.


Conclusion

Our results suggest that HLA class II antigens are commonly expressed in precancerous stages and cervical cancers. The low percentage of HLA class II positivity in CIN1 is compatible with the hypothesis that only a subset of CIN1, potentially those originating from the squamocolumnar junction zone, may overexpress HLA class II antigens and tend to progress into higher grade CIN. In later disease stages, HLA class II antigen-positive cell clones may be eliminated in an environment of dense T cell infiltration, which would be compatible with the immunoediting concept.


References