Human Papillomavirus (HPV) is a well-studied etiologic agent for cervical cancer. The HPV test, as primary method of cervical cancer screening, decreases the incidence of invasive carcinoma, and its performance is superior when compared to cytology.
The aim of this study was emphasizing the overall performance of the methods used by CML-GS and correlate the results with cytological examinations in a sample population from HCD.
From January-2012 to Decemeber-2015 were analyzed 4685 cervical samples by HPV-molecular and conventional-cytology methods. HPV-molecular methods used where: Hybrid-Capture2; Cobas-HPV test; Clart Human papillomavirus 2; PapilloCheck.
The cytological results were registered with SNOMED nomenclature: Normal; ASC-US; ASC-H; Glandular cell atypia; LIEBG; LIAG and Squamous cell carcinoma
Hybrid-Capture2:
1. 425 (26.02%) as HPV-HR positive and 1208 (73.97%) as HPV-HR negative.
2. detection-rate 54.92%; 83.88% specificity; 12.00% FPR; false-negative-rate of 11.51%.
Cobas-HPV test:
1. 631 (22.19%) as HPV HR positive and 2213 (77.81%) as HPV HR negative.
2. detection-rate 59.12%; 84.85% specificity; 12.73% FPR,; false-negative-rate of 6.54%.
Genotyping-HPV test:
1. 105 (47.51%) as HPV HR positive and 116 (52.49%) as HPV HR negative.
2. detection-rate 69.14%; 65.00% specificity; 22.17% FPR,; false-negative-rate of 11.31%.
Most frequent HR-types: HPV53(11.98%), HPV16(10.78%), HPV66(10.18%), HPV51(7.78%). Multiple HPV-infections decreased by the cytological severity.
Our HPV type prevalence findings were similar from others found in populations HPV studys. The most frequent hr-HPV in Portuguese population is HPV53, where the malignancy rate is not as high as 16/18. We can expect a HPV type shift, possibility from universal vaccination.
The hc2 and 16/18-Cobas accomplished concordance in false-positive-rate, detection-rate and specificity. The statistically significant differences (p-value<0.05) are 16/18-Cobas yield lower false-negative-rate for Abnormal Cytological results, subsequently higher negative-predictive-value, induce 16/18-Cobas testing better for triage.