OC 15-07ANALYSIS OF HUMAN PAPILLOMAVIRUS TYPE-16 AND -18 LINEAGES IN IRANIAN WOMEN BASED ON LONG CONTROL GENE REGION

02. Epidemiology and natural history
S.A. Nadji 1, M. Mobini Kesheh 2, M. Barazandeh 2, S. Sharif Kashani 2, S. Mirab Samiee 3, A. Motlagh 4.
1Virology Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran. (Iran, Islamic Republic of), 2National HPV reference Laboratory, Tehran, Iran. (Iran, Islamic Republic of), 3Reference Health Laboratory, Ministry of Health and Medical Education, Iran. (Iran, Islamic Republic of), 4Cancer Office, Non-Communicable Diseases, Deputy of Health, Ministry of Health and Medical Education, Tehran, Iran (Iran, Islamic Republic of)

Background / Objectives

Both HPV-16 and HPV-18 play a prominent role in the development of cervical cancer and it was suggested that some variants of HPV-16 and HPV-18 might confer differential risk of cervical disease. The genetic lineages of HPV16 and 18 are not known in Iran. The purpose of this study was to analyze variations of HPV-16 and -18 lineages within the LCR region.


Methods

A total of 2756 samples; including 2638 Cervical ThinPrep and 118 vaginal swabs from Iranian females of 12 provinces, were subjected to HPV screening and then genotyping. Totally 56 samples became positive for HPV-16 (41 samples) and HPV-18 (15 samples). To analyze the HPV lineages, a partial nucleotide sequence of LCR region was sequenced and phylogenetic and SNP analysis were performed using MEGA 5.05 software and HPV genome references.   


Results

Sequence analysis of HPV-16 showed that sub-lineage NA1 are the major variant (81.6%, n=31). After NA, sub-lineage EP1 (15.8%, n=6) and the only African variant (AFR2a) were followed. 10 new SNPs were observed in studied HPV-16 sequences. Two new SNPs, T7436G and C7782T, were observed in most of isolates under sub-lineage NA. Eu lineage of HPV-18 is the most prevalent variant in Iran (92.3%, n-12), that followed by As-Am. No AF variant of HPV-18 was found in our study.

 

 


Conclusion

We now report the distribution of HPV16 and -18 variants in Iran. Despite other Asian countries, Non-Asian HPV-16 and HPV-18 variants are prevalent in Iran. A possible reason of low incidence of cervical cancer in Iranian women could be explained by this observation.


References