Human papillomaviruses (HPV) cause precancerous lesions and cancers of the cervix. In Côte d'Ivoire, cervical cancer screening program based on visual inspection is the gold standard. This study aims to detect High risk (HR) HPV DNA on women attending for cervical cancer screening program based on visual inspection after application of acid acetic then lugol
From March to December 2015, endocervical samples from women attending cervical screening were tested for some HR-HPV. HPV DNA was amplified using PGMY09 /11 primers which generated 450 base pairs at the L1 region. The samples harboring HPV DNA were genotyped using the multiplex PCR with HPV 16, 18, 31, 33, 35, 45 and 51 primers.
The mean age of this population was 32 years. On 339 women enrolled on visual inspection 6.19% were positive. HPV DNA was obtained in 9.73 of the population. Thirty-one of 33 samples (93.93.%) of HPV DNA+ were genotyped using multiplex PCR testing for HPV 16,18, 31, 33, 35, 45 and 51 Of those women with HPV DAN+. 28.57% had a single infection while 71.43% had a multiple infection. HPV genotypes prevalence were the followed: HPV 16 (30.00%), HPV 18 (25.00%), HPV35 (20.00%), HPV 45 (20.00%), HPV 51 (3.30%) and HPV 33 (1.60%). By using PCR as gold standard VIA sensitivity was 16.12% and specificity 95.45 %
HPV prophylactic vaccine would prevent 33.33% of HR HPV infection with the 2v, 33.33% with the 4v and 66.66 % with the 9v vaccines respectively. In Cote d'Ivoire screening for cervical cancer with HR HPV testing and triaging for treatment with visual inspection would represent a very efficient prevention of cervical cancer program.
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