Head and Neck Squamous Cell Carcinoma (HNSCC) is a malignant disease entity with a high prevalence in the world population (1). Among its major risk factors, there is a persistent infection with Human Papilloma Virus (HPV), which has been related to a better prognosis in patients. HPV infection results in an immunoreactivity of p16 protein that has been used as a marker of the oncogenic lineage by this etiologic agent (3,4). Objective: To analyze epidemiological aspects of patients affected by HNSCC (age, sex and location of the lesion), and relate them to the prevalence of HPV infection. To evaluate the presence of virus stigmas the samples (koilocytes). To correlate histological types and differentiation of HNSCC positive for p16
A cross-sectional and retrospective study in the electronic archives of the Hospital Universitário Evangélico de Curitiba, in cases with diagnosis of Oropharyngeal SCC, which occurred between January 2005 and December 2015. Slides stained by the HE technique were reviewed and classified histological type of the lesion and and verifiedof histological stigmas characteristic of HPV (koilocytes). Squamous cell carcinomas (SCC) were classified in keratinizing ,no keratinizing and miixed The paraffin blocks were screened to select the sample areas for the preparation of tissue microarrays (TMAs) in which was performed immunohistochemical study of the p16 protein by avidin-biotin technique. All results and information obtained were tabulated according to data protocol, and then expressed through graphs and tables and statistical analysis was performed by parametric and non-parametric methods, with significance of p <0.05 . The project, was approval by the Research Ethics Committee of the Evangelical Society of Paraná
Of the 51 cases evaluated, 42 were males and 9 females, mean age of 61 years. There was a higher percentage of tumors affecting the larynx (43%), with higher prevalence of keratinized cancers on non keratinized. Koilocytosis was observed in 56.9% of cases, and immunostaining for p16 was 49.02%, predominantly in tumors not keratinized (p = 0.03532).
The present study has demonstrated that the infection prevalence of HPV in HNSCC, through the immunostaining with p16, was present in 49.02% of cases. Toward the epidemiological profile, carcinomas were more common in male individuals with middle ages of 61 years and in the larynx as more often topography. Koilocytosis was found in 29 cases, corresponding to 56.86% of our sample. The immunoreactivity of p16 protein predominated im non keratinized tumors.
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