P24-02ETIOLOGICAL ROLE OF HUMAN PAPILLOMAVIRUS INFECTION IN THE DEVELOPMENT OF PENILE CANCER cer

24. Vulvar diseases and neoplasia
J. Sakamoto 1, K. Shigehara 1, K. Nakashima 1, S. Kawaguchi 1, T. nakashima 2, M. shimamura 3, M. Yasuda 4, T. Hasegawa 5, Y. Kobori 6, H. Okada 7, T. Deguchi 8, M. Namiki 5, A. mizokami 1.
11) Department of Urology, Kanazawa University Graduate School of Medical Science (Japan), 22) Department of Urology, Ishikawa Prefectural Central Hospital (Japan), 33) Department of Urology, Nomi City Hospital (Japan), 44) Department of Urology, Gifu University Hospital (Japan), 55) Hasegawa Hospital (Japan), 66) Department of Urology, Dokkyo Medical School Koshigaya Hospital (Japan), 76)Department of Urology, Dokkyo Medical School Koshigaya Hospital (Japan), 84)Department of Urology, Gifu University Hospital (Japan)

Background / Objectives

We investigated an etiological role of HPV infection in the development of penile carcinoma.

 


Methods

Paraffin-embedded tumor samples were collected from 17 patients who had received an operation for penile carcinoma. After DNA extraction from each sample, HPV-DNA test and genotyping were performed using a HPV GenoArray kit (Hybri MaxTM). In addition, localization of HPV was observed by in situ hybridization(ISH) for high-risk HPV-DNA. Furthermore, P16-INK4a and HPV-L1 capsid protein expression were evaluated by immunohistochemistry (IHC).


Results

HPV-DNA was detected in 7 (41%) cases; HPV16 was identified in 5 samples, HPV33 and HPV68 was identified in one case, respectively. ISH analysis demonstrated that high-risk HPV-DNA was localized with punctate staining patterns in the nuclei of tumor cells of all HPV-positive samples. P16-INK4a was moderately to strongly expressed in nuclei and cytoplasma of tumor cells in many of HPV-positive samples, whereas showed the relatively weak or no expressions in HPV-negative ones. On the other hand, HPV-L1 protein expression, which suggested reproductive HPV infection, was not observed in any carcinoma.


Conclusion

The current results suggest that high-risk HPV, especially HPV16 is likely to be a causative agent among an approximate 40% of the Japanese patients with penile carcinoma, although further studies including a large number of samples are required to reach a more definite conclusion.


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