P17-01THE INTERACTION BETWEEN HPV INFECTION AND BACTERIAL MICROBIOTA IN PLACENTA, CERVIX AND ORAL MUCOSA

17. Microbiome
H. Tuominen 1, M.C. Collado 2, S. Syrjänen 3, S. Rautava 4, J. Rautava 3.
1Department of Oral Pathology and Oral Radiology, Institute of Dentistry, Faculty of Medicine, University of Turku, Turku, Finland (Finland), 2Department of Biotechnology, Institute of Agrochemistry and Food Science, Spanish National Research Council (IATA-CSIC), Valencia, Spain (Spain), 3Department of Oral Pathology and Oral Radiology, Institute of Dentistry, Faculty of Medicine, University of Turku, Turku, Finland & Department of Pathology, Turku University Hospital, Turku, Finland (Finland), 4Department of Paediatrics, University of Turku & Turku University Hospital, Turku, Finland (Finland)

Background / Objectives

Objective. We aimed to investigate whether an existing HPV infection has influence on the bacterial microbiota composition in the placenta and cervix as well as in the maternal and infant oral mucosa.


Methods

Materials and methods. This study is a nested case-control study based on samples collected in the prospective Finnish Family HPV Study. Total 39 families were selected for this study based on placenta HPV status, the mode of birth and availability of samples (13 cases with HPV positive placenta and 26 controls with HPV negative placenta of which 13 were obtained through vaginal delivery and 13 by Caesarean section). The corresponding maternal cervical and oral and infant oral samples were selected for analyses.

HPV DNA genotyping of 24 different genotypes (6 low-risk and 18 high-risk types) of the samples was conducted using Multimetrix ® assay (Multimetrix, Regensburg, Germany). Microbiota composition and diversity was characterized by 16S rRNA gene sequencing (V1-V3 region, Illumina protocol, Illumina, San Diego, CA, USA). 


Results

Results. HPV DNA was found in 23% (9/39) maternal cervix, 33% (13/39) maternal oral, and 45% (18/40, included one set of twins) infant oral samples. HPV16 was the most frequent type found in all groups studied (54% of placenta, 22% of cervix, 54% of maternal oral and 39% of infant oral samples).

In maternal mouth, HPV positive samples displayed significantly higher richness (Chao1 index) of bacterial microbiota (p=0.032) but no difference in Shannon index. HPV status did not influence microbial diversity and richness in the other samples.

The HPV positive cervix harboured significantly more Adlecreutzia (p=0.048), Mycoplasma (p=0.048) and Gemella (p=0.0058) genus as compared to HPV negative cervical samples. In maternal oral samples, Selenomonas spp. was significantly increased (p=0.012) in HPV positive individuals whereas the amount of Propionibacterium (p=0.026) and Staphylococcus (p=0.049) were increased in HPV positive infant oral samples. In the placenta, Lactobacillus (p=0.076) were slightly increased in HPV positive samples compared to placenta HPV negative.


Conclusion

Conclusion. HPV infection is associated with altered bacterial microbiota composition in the placenta and mouth. Whether the changes in bacterial microbiota predispose or result from HPV remains to be determined in future studies. 


References