P12-04PROTEOMIC COMPOSITION OF CERVICOVAGINAL FLUID IN HPV- ASSOCIATED CERVICAL LESIONS
12. Molecular markers
V. Frankevich 1, N. Nazarona 2, M. Zardiashvili 3, M. Nekrasova 3, A. Bugrova 4, A. Kononikhin 5, A. Brzhozovsky 6, N. Starodubtseva 7, V. Prilepskaya 8, G. Sukhikh 9.
1PhD, Head of Department of Systems Biology in Reproduction, Federal State Budget Institution “Research Center for Obstetrics, Gynecology and Perinatology” Ministry Of Healthcare of the Russian Federation (Russian Federation), 2MD, PhD, Senior Research Fellow Federal State Budget Institution “Research Center for Obstetrics, Perinatology” Ministry Of Healthcare of the Russian Federation (Russian Federation), 3PhD student Federal State Budget Institution “Research Center for Obstetrics, Gynecology and Perinatology” Ministry Of Healthcare of the Russian Federation (Russian Federation), 4PhD, Senior Researcher of Proteomics of Human Reproduction, Federal State Budget Institution “Research Center for Obstetrics, Gynecology and Perinatology” Ministry Of Healthcare of the Russian Federation (Russian Federation), 5PhD, Researcher of Proteomics of Human Reproduction, Federal State Budget Institution “Research Center for Obstetrics, Gynecology and Perinatology” Ministry Of Healthcare of the Russian Federation (Russian Federation), 6PhD student of Laboratory of proteomics, Institute of Biomedical Problems – Russian Federation State Scientific Research Center, Russian Academy of Sciences, (Russian Federation), 7PhD, Head of Laboratory of Proteomics of Human Reproduction, Federal State Budget Institution “Research Center for Obstetrics, Gynecology and Perinatology” Ministry Of Healthcare of the Russian Federation (Russian Federation), 8MD, PhD, Professor, Deputy Director for Science, Federal State Budget Institution “Research Center for Obstetrics, Gynecology and Perinatology” Ministry Of Healthcare of the Russian Federation (Russian Federation), 9Academician of RAMS, MD, PhD, Professor, Director, Federal State Budget Institution “Research Center for Obstetrics, Gynecology and Perinatology” Ministry Of Healthcare of the Russian Federation (Russian Federation)
Background / Objectives
To determine changes of the cervicovaginal fluid (CVF) proteomic composition for assessment of the severity of HPV-associated cervical lesions among reproductive age women.
Methods
The study involved 30 women with various forms of HPV-associated cervical lesions (ASCUS, LSIL and HSIL). All samples of cervicovaginal fluid were prepared for further proteomic analysis by tandem mass spectrometry (HPLC-MS/MS). Semi-quantitative data analysis including identification and annotation of proteins was carried out using the software package MaxQuant and Perseus.
Results
The protein panels specific to the various forms of HPV-associated cervical lesions (ASCUS, LSIL and HSIL) were identified. The first group of proteins (P4HB, HSPA8, C4BPA and others) characterized the early changes associated with HPV infection and cervical epithelium lesion, including penetration of viruses into the cell and its transcription, impaired function of the complement system. The second group of proteins (PRDX5, YWHAE, LRG1 and others) were directly involved in the development and progression of cervical neoplasia and characterized late changes, in particular, reduced apoptosis, impaired differentiation and maturation of the epithelium, and the transformation of atypical cells.
Conclusion
The protein composition of the CVF was studied to assess the severity of HPV- associated cervical epithelial lesions in reproductive age patients by tandem chromato-mass spectrometry (HPLC-MS / MS). The protein panels, specific for various forms of HPV-associated cervical epithelial lesions are determined (the first group - P4HB, HSPA8, C4BPA, the second group - PRDX5, YWHAE, LRG1).