FC 12-15RISK ALLELIC LOAD IN TH2 AND TH3 CYTOKINES GENES AS BIOMARKER OF SUSCEPTIBILITY TO HPV-16 POSITIVE CERVICAL CANCER: A CASE CONTROL STUDY

12. Molecular markers
K. Torres-Poveda 1, M. Bahena-Román 1, R. Méndez-Martínez 2, A. Zurita-Díaz 1, K. Delgado-Romero 3, D. Cantú 4, A. García-Carrancá 4, V. Madrid-Marina 1.
1Chronic Infectious Diseases and Cancer Division. Center for Research on Infectious Diseases. Instituto Nacional de Salud Pública, Cuernavaca, Morelos, Mexico (Mexico), 2Division of Basic Research, Instituto Nacional de Cancerología (INCan),Mexico City, Mexico (Mexico), 3Centro de Atención para la Salud de la Mujer (CAPASAM). (Center for Women’s Health). Health Services of the State of Morelos, Cuernavaca, Morelos, Mexico (Mexico), 4Unit of Biomedical Research in Cancer, Instituto Nacional de Cancerología (INCan), Mexico City, Mexico (Mexico)

Background / Objectives

Background: Alterations in the host cellular immune response allow persistent infections with High-Risk Human Papillomavirus (HR-HPV) and development of premalignant cervical lesions and cervical cancer (CC). Variations of immunosuppressive cytokine levels in cervix are associated with the natural history of CC. Objectives: To assess the potential role of genetic host immunity and cytokines serum levels in the risk of developing CC, we conducted a case–control study paired by age.


Methods

Methods: Peripheral blood samples from patients with CC (n = 200) and hospital controls (n = 200), were used to evaluate nine biallelic SNPs of six cytokine genes of the adaptive immune system by allelic discrimination and cytokines serum levels by ELISA.


Results

 

Results: After analyzing the SNP association by multivariate logistic regression adjusted by age, CC history and smoking history, three Th2 cytokines (IL-4, IL-6 and IL-10) and one Th3 (TGFB1) cytokine were significantly associated with CC. Individuals with at least one copy of the following risk alleles: T of SNP (−590C > T IL-4), C of SNP (−573G > C IL-6), A of SNP (−592C > A IL-10), T of SNP (−819C > T IL-10) and T of SNP (−509C >T TGFB1), had an adjusted odds ratio (OR) of 2.08 (95 % CI 1.475–2.934, p = 0.0001), an OR of 1.70 (95 % CI 1.208–2.404, p = 0.002), an OR of 1.87 (95 % CI 1.332–2.630, p = 0.0001), an OR of 1.67 (95 % CI 1.192–2.353, p = 0.003) and an OR of 1.91 (95 % CI 1.354–2.701, p = 0.0001), respectively, for CC. The burden of carrying two or more of these risk alleles was found to have an additive effect on the risk of CC (p trend = 0.0001). Finally, the serum levels of Th2 and Th3 cytokines were higher in CC cases than the controls; whereas IFNG levels, a Th1 cytokine, were higher in controls than CC cases.


Conclusion

 

Conclusion: The significant associations of five SNPs with CC indicate that these polymorphisms are potential candidates for predicting the risk of development of CC, representing a risk allelic load for CC and can be used as a biomarker of susceptibility to this disease1.


References

(Reference: 1Torres-Poveda K, Burguete-García AI, Bahena-Román M, Méndez-Martínez R, Zurita-Díaz MA, López-Estrada G, Delgado-Romero K, Peralta-Zaragoza O, Bermúdez-Morales VH, Cantú D, García-Carrancá A, Madrid-Marina V. Risk allelic load in Th2 and Th3 cytokines genes as biomarker of susceptibility to HPV-16 positive cervical cancer: a case control study. BMC Cancer 2016;16(1):330. doi: 10.1186/s12885-016-2364-4).