Study of diagnostic significance of methylation status of WIF1 gene and microRNA expression (Mir 92a, 22, 25) in diagnosis of SIL and cervical cancer.
Clinical (including colposcopy); fluid cytology with agent staining using ВD Sureph T.M. method for immunocytochemical (ICC) examination with p16/Ki67 dual labeling; HPV test by means of RT-PCR method, WIF1 gene methylation; microRNA expression (Mir 92a, 22, 25). Final diagnostic verification of SIL and cervical cancer has been carried out on the basis of histologic examination.
101 patients aged from 18 to 49 years have been tested (average age 32.7±0.5 years).
The 1st group – 31 patients with LSIL; the 2nd group – 26 patients with HSIL; the 3rd group – 12 patients with squamous cervical cancer (SCC); the 4th group – 32 healthy patients negative for intraepithelial lesions or malignancy (NILM).
Correlation analysis of morphological examination and cancer markers p16 and Ki67 has shown direct, strong and significant correlation between these two methods (r- Pearson = + 0.7 p = 4,75 × 10‑17 for Ki67; r-Pearson = + 0,83; p = 1,8 ×10‑29 for р16).
Correlation analysis between the findings of morphological examination and WIF1 gene methylation has established direct, strong, significant bivariate correlation between these two methods (r-Pearson= + 0,8; p = 7,0 ×10‑33), besides correlation analysis between microRNA expression has demonstrated direct, significant correlation between MiR92a (r-Pearson = + 0,27; p=0,007).
We have revealed significant correlations between the findings of morphological examination confirming the diagnosis of SIL and squamous cervical cancer; cancer markers p16 and Ki67; WIF1 gene methylation; and microRNA expression. All above-mentioned methods can be used in complex diagnosis of SIL and cervical cancer.