E-poster - Blood Processing, Storage and Release

• P-209 • STORAGE OF LEUKOREDUCED RED CELLS AS A SAFE ALTERNATIVE TO IRRADIATION > C. Carly Olafson
Objectives : 1. Importance of using irradiation to mitigate the risk of TA-GvHD and how irradiation impacts quality of red cell concentrates
2. The impact of leukocyte filtration on the viability of residual white cells in red cell concentrates
3. Hypothermic storage of red cell concentrates as an alternative to irradiation to reduce the risk of TA-GvHD • P-210 • COLD-STORAGE OF PLATELET COMPONENTS INDUCES CHANGES IN RECEPTOR EXPRESSION ASSOCIATED WITH INNATE IMMUNE FUNCTION > B. Ben Wood
Objectives : - Platelets can facilitate immune function
- Platelet refrigeration reduces the surface expression of toll-like receptors and several receptors associated with platelet leukocyte interaction
- These changes may alter how refrigerated platelets interact with pathogen associated molecular patterns (PAMPs), damage associated molecular patterns (DAMPs) and leukocytes
• P-211 • EFFECT OF X-IRRADIATION AT LOWER AND HIGHER IRRADIATION DOSES ON RED CELL CONCENTRATE QUALITY > A. Athinoula MELI
Objectives : Red cell concentrates x-irradiated at higher doses than 36Gy show increased haemolysis levels, increased potassium release and lower ATP levels.
On the contrary, lower x-irradiation doses than 36Gy improve red cell concentrates storage quality.
Red cell concentrates x-irradiated up to 72Gy dose are of acceptable quality by the end of shelf life. • P-212 • PLATELET AUTOPHAGIC ACTIVITY AS A COMPONENT OF STORAGE LESION > S. Sebastien FAUTEUX-DANIEL • P-214 • PHYSIOLOGICAL AND METABOLICAL NETWORK ANALYSIS OF STORED RED BLOOD CELLS FROM ELIGIBLE DONORS WITH OR WITHOUT BETA-THALASSEMIA TRAITS > A. Alkmini ANASTASIADI
Objectives : 1) Definition of central parameters in the physiology of stored red blood cells from beta-thalassemia heterozygous donors.
2) Hints on the effect of baseline physiology to stored red blood cell quality.
3) Presentation of the first fresh/stored red blood cell interactome of beta-thalassemia heterozygous donors. • P-215 • NON-DESTRUCTIVE QUALITY ASSESSMENT OF LEUKOREDUCED WHOLE BLOOD USING MINI-BAGS > P. Peter SCHUBERT • P-216 • METABOLIC PARAMETERS AND AGGREGATION OF STORED PLATELETS AT THREE DIFFERENT CONDITIONS > P. Puneeth Babu ANNE • P-218 • ADDITIVE SOLUTION REPLACEMENT DOES NOT IMPROVE THE QUALITY OF RED BLOOD CELL CONCENTRATES > A. Agnieszka ZEBROWSKA • P-219 • IMPROVED FUNCTIONAL AND BIOCHEMICAL CHARACTERISTICS OF BUFFY-COAT PLATELET CONCENTRATES STORED IN CITRATE-FREE PAS-III ADDITIVE SOLUTION FOR 7 DAYS > B. Beatrice HECHLER
Objectives : -evaluate the impact of citrate removal from PAS-III additive solution on platelet metabolism during storage of BC-PCs for 7 days
-evaluate the impact of citrate removal from PAS-III additive solution on platelet activation of BC-PCs for 7 days
-evaluate the impact of citrate removal from PAS-III additive solution on platelet function during storage of BC-PCs for 7 days • P-220 • STORAGE OF HYPOXIC RED BLOOD CELLS IN NON-DEHP STORAGE BAGS FOR 42 DAYS > S. Samuel SOWEMIMO-COKER
Objectives : 1) To determine whether hypoxic red blood cells can be stored in Non DEHP PVC bags plasticized with DINCH and BTHC with maintenance of red cell hemolysis below 0.8% at the end of 42 day refrigerated storage
2) To compare the properties of hypoxic red blood cells with normoxic red blood cells
3) To demonstrate the benefits of hypoxic storage conditions in preserving the key metabolites such as ATP and 2,3DPG • P-221 • CLOTTING ACTIVITIES OF FRESH FROZEN PLASMA THAWED BY A NEWLY DEVELOPED DRY-THAWING DEVICE > K. Kazuhiko IKEDA
Objectives : 1. Recently, a new dry tempering system, FP-2000 (Hokuyo Denki, Kitamoto, Japan) has been developed as a new device to thaw FFP.
2. FP-2000-thawed FFP showed similar clotting activities as FFP thawed by a water-bath system.
3. Availability without requirement of water heating may help immediate preparation of FFP and to avoid bacterial contamination via water. Therefore, FP-2000 may provide a good option to thaw FFP.
• P-222 • ASSESSMENT OF SICKLE CELL TRAIT AS A FACTOR OF FILTRATION FAILURES IN TURKEY > N. Nazli SOZMEN • P-224 • BUFFY COATS CAN BE AGITATED OVERNIGHT BEFORE A SOFT SPIN TO PREPARE PLATELET CONCENTRATE > C. Chunyan YAO • P-225 • INNOVATION FOR BLOOD CENTERS AND CELL THERAPY UNITS: ACOUSTIC CELL PROCESSING > D. Déborah FRANCOIS • P-226 • IMPACT OF DONOR EXPOSOME ON STORED RED BLOOD CELL METABOLISM > A. Angelo D’ALESSANDRO • P-227 • IN VITRO QUALITY EVALUATION OF LEUCODEPLETED PLATELET CONCENTRATES PREPARED IN ADDITIVE SOLUTION SSP+ FROM BUFFY-COAT POOLING. PUEBLA, MEXICO 2019 > D. Dalia MORENO
Objectives : 1. To describe the characteristics of platelets concentrates obtained by BC pooling stored up to 7 days in additive solution for platelets (SSP+)
2. To display the possibility of the validation of a different method to process platelet concentrate in an important blood bank in Mexico
3. To indicate the in-vitro quality of the leucodeplete platelet concentrates validated up to 7 days of storage. • P-228 • A COMPARISON OF X-RAY AND GAMMA IRRADIATION: EFFECTS ON THE IN VITRO QUALITY OF STORED RED CELL AND PLATELET COMPONENTS AND EFFICACY OF LYMPHOCYTE INACTIVATION > D. Denese MARKS
Objectives : - Describe the effects of irradiation on red cell components
- Describe the effects of irradiation on platelet components
- Describe the effects of irradiation on lymphocytes • P-229 • HYPOXIC STORAGE IMPROVED THE QUALITY AND VISCOSITY OF RED BLOOD CELLS IN PAGGSM ADDITIVE SOLUTION > S. Samuel SOWEMIMO-COKER
Objectives : 1) To demonstrate the effects of gamma irradiation on the quality of hypoxic and normoxic red blood cells
2) To demonstrate the effects of hypoxic storage on the metabolic activities of gamma irradiated red blood cells
3) To compare the viscoelastic properties (Red cell aggregation and deformability) gamma-irradiated hypoxic red blood to that of conventionally stored red blood cells • P-230 • THE SURFACE MARKERS AND SURVIVAL RATE OF PLATELETS DURING STORAGE AT 4°C: THE INFLUENCE OF SODIUM OCTANOATE > V. Vahid BAGHDADI • P-231 • LEUKODEPLETED WHOLE BLOOD (LWB) FOR MASSIVE TRAUMA PATIENT. PRODUCT QUALITY AND IMPLEMENTATION AT THE EFS > S. Stéphane BÉGUÉ
Objectives : Stéphane Bégué (MS/MSc) is the National Head of Department for Quality Control and Evaluation of Blood Components at the Medical Direction at EFS headquarters (French National Blood Operator). Responsible for National evaluation studies of blood components and processes, in charge of the Quality Control Laboratories of the 13 Regional Blood Banks of the EFS, to ensure safety and quality of Labile Blood Products. ICA Lead Auditor.
In transfusion since 1988, his experience in transfusion technology covers plasma fractionation, PDMPs, blood components processing technology, Implementation of Quality System and Quality Control of blood components, Quality audit (first and second party), Blood transfusion IT systems, Blood processing technology. Active contributing member of EBA, EDQM, SFTS.
• P-232 • HOW TO GUARANTEE SUPPLY OF SAFE BLOOD IN CASTILLA-LEON USING AUTOMATION AND PRT > M. Marcia CARDOSO • P-233 • ANALYSIS OF BIOCHEMICAL PARAMETERS OF TWO ADDITIVE SOLUTIONS FOR PLATELET CONCENTRATES: DO THEY ACCOMPLISH REQUISITES FOR ADEQUATE STORAGE? > A. Angel Luis PAJARES HERRAIZ
Objectives : PAS composition
Storage of CP
Metabolic effect of PAS • P-234 • CRYOPRESERVED PLATELETS : A FEASIBILITY STUDY IN RESOURSE LIMITED SETTING AND COMPARISON OF PLATELET ADDITIVE SOLUTION / PLASMA FOR RECONSTITUTION > P. Priti ELHENCE • P-235 • METABOLOMICS ANALYSIS OF STORED RBCS FROM DONORS WITH BETA-THALASSEMIA TRAITS > V. Vasileios TZOUNAKAS
Objectives : 1) Indications for different glycolytic rates in fresh blood from beta-thalassemia heterozygous donors.
2) Differential metabolism in terms of purine and arginine pathways in stored red blood cells from donors with beta-thalassemia traits.
3) Information about the unique hexosamine profile of the same red blood cells during storage in blood bank conditions. • P-236 • IRRADIATING STORED BLOOD OR STORING AN IRRADIATED BLOOD: IS IT DIFFERENT? – A STUDY OF SERIAL CHANGES IN POTASSIUM AND LACTATE LEVELS > A. Abhishekh BASAVARAJEGOWDA
Objectives : Irradiation leads to increased Potassium and lactate levels in the supernatant of PRBC units
Irradiation and storing PRBC units leads to more detrimental storage lesions compared to Irradiating an already stored bag
Irradiation as close to the issue of blood products is better in terms of quality compared to storing preirradiated PRBCs • P-238 • EVALUATION OF THREE BUFFY COAT BASED METHODS OF BLOOD COMPONENT PREPARATION IN TOP AND BOTTOM BLOOD BAG CONFIGURATION > D. Dheeraj KHETAN • P-239 • THE EFFECT OF IRRADIATION ON THE POTASSIUM LEAKAGE FROM RED CELLS FROM INDIVIDUALS WITH FAMILIAL PSEUDOHYPERKALAEMIA > A. Athinoula MELI
Objectives : Both irradiation and Familial Pseudohyperkalaemia (FP) cause increased intracellular potassium release from cold-stored red cells.
When irradiated, red cell concentrates from FP individuals do not release higher potassium levels by the end of storage compared to irradiated red cell concentrates from individuals without FP and no other red cell storage parameters are altered.
• P-240 • VALIDATION OF THE SEMI-AUTOMATED SEPARATION SYSTEM FRACTIOMATIC X2 (GRIFOLS®) WITH LEUCORED T&B BAGS AND ASAHI R-S11 FILTER FOR THE OBTENTION OF BLOOD COMPONENTS > A. Angel Luis PAJARES HERRAIZ
Objectives : Obtention of Blood Components with semiautomated separators
Standaritation with semiautomated separators
Comparation of two leucocyte filters • P-241 • ASSESSMENT OF HEMOLYSIS OF RED BLOOD CELL PRODUCTS DELIVERED BY PNEUMATIC TUBE SYSTEM > I-C. I-Chien HSIEH
Objectives : Red blood products translation
pneumatic tube system
hemolysis

• P-242 • USING DATA TO OPTIMIZE A WHOLE BLOOD PLATELET PRODUCTION PROCESS > A. Andy VOLCKAERT
Objectives : Using the T-pool select application reduces the resources (time & number of single units per pool) needed to produce the desired number of WB PCs and improves the efficiency of the WB PC production process. Using an electronic, IT based system, also minimizes the risk of human error and facilitates GMP compliance. • P-243 • EVALUATION OF THE USE OF A BLOOD COMPONENT VISUAL INSPECTION GUIDE TO IDENTIFY ICTERIC BLOOD COMPONENTS > R. Ramir ALCANTARA
Objectives : 1. Define what is Icterus
2. Determine the appearance of icteric plasma in red cell, plasma and platelet units
3. What are the problems in identifying icteric plasma • P-244 • THE ROLE OF TRANSFORMING GROWTH FACTOR-BETA 1 (TGF-ß1) AS A PREDICTOR OF STORAGE LESION IN PACKED RED CELLS (PRCS)-BLOOD PRODUCT AT SANGLAH HOSPITAL, BALI, INDONESIA: A PRELIMINARY STUDY > A. Anak Agung Wiradewi LESTARI
Objectives : Blood Transfusion; Storage Lesion; TGF-B1 • P-245 • IMPLEMENTATION OF DOUBLE SEMIAUTOMATIC FRACTIONATION SYSTEM IN THE TRANSFUSION CENTER OF CIUDAD REAL > M. Mª Del Castillo JARILLA FERNÁNDEZ
Objectives : Implantation of automatic fractionators with double fractionation system (FRACTIOMATIC X2) in the processing section of our Transfusion Center (CT) of Ciudad Real

To look for the achieve optimization and performance improvement in obtaining blood.

Guarantee the quality of blood components obtained from fractionation.
• P-247 • COMPARISON OF QUALITY AND BIOCHEMICAL ACTIVATION MARKERS AMONG BUFFY COAT POOLED PLATELETS (NON-LEUCOREDUCED VS LEUCOREDUCED VS LEUCOREDUCED WITH PLATELET ADDITIVE SOLUTION) VS APHERESIS PLATELETS. > H. Hari Krishan DHAWAN
Objectives : 1. How to design a study to compare the quality of different variations in blood component.
2. Various quality control parameters and biochemical activation markers used for testing platelet product.
3. Which platelet preparation shows best in vitro quality during storage. • P-248 • ASSESSMENT OF DIFFERENT EX-VIVO STORAGE CONDITIONS OF PLATELET CONCENTRATES ON THEIR IN-VITRO HAEMOSTATIC ABILITY AS INTERPRETED BY THROMBOELASTOGRAPHY (TEG). > P. Poonam COSHIC • P-249 • THREE BUFFY COAT POOLING IN LEUKOCYTE DEPLETED PLATELET CONCENTRATES PREPARATION, DECREASE BLOOD DONOR EXPOSURE IN BLOOD TRANSFUSION CENTRE, FACULTY OF MEDICINE, KHON KAEN UNIVERSITY, THAILAND > T. Thipaporn JAROONSIRIMANEEKUL • P-250 • QUALITY PARAMETERS AND HEMOSTATIC FUNCTION OF LEUCODEPLEETED WHOLE BLOOD - COMPARATIVE STUDY WITH WHOLE BLOOD NON-LEUCODEPLEETED > M. Miloš BOHONĚK
Objectives : For resuscitation of polytraumatic patiens with haevy bleeding is renewed interest in the use of whole blood (WB). Modern blood transfusion products should be leucodepleeted and WB should comply with this standard. Leucodepleeted WB is prepared with platelet-sparing filters, yet effect of the procedure on WB quality parameters and haemostatic function are not well characterized.
The aim of the study was to evaluate in vitro quality parameters and haemostatic function of leucodepleeted WB and their comparison with convetional non-leucodepleeted WB.
The in-vitro quality parameters and hemostatic function of leucodepleeted WB with platelet-sparing filter to compare with conventional non-leucodepleeted WB are comparable and meet quality and clinical requirements. Both groups shows identical or similar changes in storage time. • P-251 • BLOOD PROCESSING, STORAGE AND RELEASE AS PART OF THE TRANSFUSION PROGRAM IMPLEMENTATION IN NOTRE DAME DE LA SANTÉ HOSPITAL (DSCHANG, CAMEROON) > N. Natalia GARCIA LEON
Objectives : 1.- Transfusion program implementation in a developing country
2.- Transfusion security in a developing country
3.- Blood processing in a developing country • P-252 • ANALYSIS OF MICROVESICLES ISOLATED FROM STORED RBC UNITS WITH THE USE OF FLOW CYTOMETRY, CONFOCAL AND ATOMIC FORCE MICROSCOPY > A. Anna STACHURSKA
Objectives : Identification and qualitative analysis of microvesicles isolated from stored RBC units with different techniques such as: flow cytometry, atomic force microscopy and confocal microscopy • P-253 • COMPARISON OF THE QUALITY CONTROL RESULTS OF THE BLOOD COMPONENTS OBTAINED BY BUFFY-COAT METHOD WITH AUTOMATED SYSTEM. > A. Ana Isabel PÉREZ ALIAGA
Objectives : To study the differences between the blood components obtained by buffy-coat and by Reveos.
To check which one is better.
to demonstrate the difficulty in the comparison of the quality control because of the method of measurement
• P-254 • EVALUATION OF THE QUALITY OF PLATELETS FROM WHOLE BLOOD, COMPARISON OF THIS BLOOD COMPONENT PREPARED USING THE REVEOS AUTOMATED BLOOD PROCESSING SYSTEM AND THE PLATELET‐RICH PLASMA METHOD > I. Inna VILSHANIVSKA • P-255 • IMPLEMENTATION OF BUFFY COAT POOLING FOR PLATELET PREPARATION AND ITS COMPARISON WITH THE PRP POOLING STRATEGY- A PILOT STUDY > R. Rajesh SAWANT
Objectives : - Methods of pooling platelets for transfusion
- Quality parameters of Buffy coat and PRP pooled platelets
- Clinical efficacy of Buffy coat and PRP pooled platelets • P-256 • IMPACT OF DONOR CHARACTERISTICS ON QUALITY OF PACKED RED CELL CONCENTRATE > R. Ravneet KAUR
Objectives : Donor characteristics does effect the quality of packed red cells
Donor characteristics does have effect on the total hemoglobin content of the packed red blood cells
Our study observed strong positive correlation between hemoglobin of blood donor , volume of blood collected and total hemoglobin content of the blood unit; Moderate positive correlation between hematocrit of blood unit and total hemoglobin content of the blood unit; Weak positive correlation between age of blood donor, Frequency of blood donors and total hemoglobin content of the blood unit.
• P-257 • IMLEMENTATION OF COMPOCOOL SYSTEM FOR WHOLE BLOOD COOLING AND TRANSPORTATION > G. Goran ANDONOV • P-258 • EVALUATION OF CLINICAL & LABORATORY EFFICACY OF PLATELET ADDITIVE SOLUTION FOR SDP TRANSFUSIONS > A. Ankit MATHUR
Objectives : 1. Platelet Additive solution helps in inventory management.
2. Clinical efficacy of PAS SDP is equivlaent to Plasma SDP.
3. Quality Parameters tested for PAS SDP found satisfactory • P-259 • EFFECT OF STORAGE TEMPERATURE 4C AND 22C ON THE QUALITY AND VIABILITY OF PLATELET CONCENTRATES BY PLATELET RICH PLASMA METHODS > M. Maya Anggun SURI • P-419 • A NOVEL BACTERIAL CULTURE SAMPLING DEVICE FOR POOLED PLATELETS > F. Fernando MARTINEZ